研究室課題ブラインドタッチ

(A high level of LDL-cholesterol is a major risk factor for development of coronary heat disease.)

A high level of LDL-cholesterol is a major risk factor for development of coronary heat disease.

(LDL-cholesterol level has been estimated indirectly from measurements of total cholesterol, triacylglycerol,)

LDL-cholesterol level has been estimated indirectly from measurements of total cholesterol, triacylglycerol,

(and HDL-cholesterol by means of the Friedewald equation in most clinical laboratories.)

and HDL-cholesterol by means of the Friedewald equation in most clinical laboratories.

(The Friedewald equation was verified by comparing its results with those of a)

The Friedewald equation was verified by comparing its results with those of a

(reference method known as beta-quantification, in which total LDL mass is directly measured)

reference method known as beta-quantification, in which total LDL mass is directly measured

(by analytical ultracentrifugation. LDL-cholesterol concentration derived by use of the)

by analytical ultracentrifugation. LDL-cholesterol concentration derived by use of the

(Friedewald equation is invalid when sample are collected in the non-fasting state or in the)

Friedewald equation is invalid when sample are collected in the non-fasting state or in the

(presence of increased triacylglycerol levels. The beta-quantification method requires)

presence of increased triacylglycerol levels. The beta-quantification method requires

(complicated instrumentation including ultracentrifugation and polyanionprecipitation steps,)

complicated instrumentation including ultracentrifugation and polyanionprecipitation steps,

(and is not available for routine use in most laboratories. However, a new and relatively simple)

and is not available for routine use in most laboratories. However, a new and relatively simple

(analytical method called N-geneous LDL-cholesterol assay was developed by Rifai et al. The)

analytical method called N-geneous LDL-cholesterol assay was developed by Rifai et al. The

(results obtained with this method correlated highly with those of beta-quantification assay,)

results obtained with this method correlated highly with those of beta-quantification assay,

(and met the established analytical performance goals recommended by the National)

and met the established analytical performance goals recommended by the National

(Cholesterol Education Program. Furthermore, LDL-cholesterol values obtained with the N-)

Cholesterol Education Program. Furthermore, LDL-cholesterol values obtained with the N-

(geneous assay are not affected by the presence of increased levels of triacylglycerol, unlike the)

geneous assay are not affected by the presence of increased levels of triacylglycerol, unlike the

(Friedewald equation, Since the Friedewald equation has still considerable advantages)

Friedewald equation, Since the Friedewald equation has still considerable advantages

(including simplicity and lack of cost. It seemed worthwhile to perform a detailed validation)

including simplicity and lack of cost. It seemed worthwhile to perform a detailed validation

(study of the Friedewald equation utilizing data obtained with the N-geneous assay as)

study of the Friedewald equation utilizing data obtained with the N-geneous assay as

(reference values.)

reference values.